2-Ethynyl-ATP (2-EATP)

Jena Bioscience

2-Ethynyl-ATP (2-EATP)

5

Research Use Only

10-11 mM

≥90%

C12H16N5O13P3

531.20 g/mol

2-Ethynyl-labeled adenosine triphosphate (2-EATP) is suitable for in vitro polyadenylation of RNA with recombinant poly(A) polymerase[1]. The resulting Alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed Azide-Alkyne click chemistry (CUAAC) that offers the choice to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)to crosslink the RNA to azide-functionalized biomolecules e.g.proteins Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

[C@@H]1([C@@H](O[C@@H]([C@H]1O)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1cnc2c(nc(nc12)C#C)N)O

-20°C

12352200