2-Fluoro-ATP, (2F-ATP) [1492-62-2]

Jena Bioscience

2-Fluoro-ATP, (2F-ATP) [1492-62-2]

5

Research Use Only

Figure 1A: Schematic representation of the secondary structure of the Gswapt. The stems are named in blue and the loops are named in green. All fluorinated bases are labeled in red. Figure 1B: 10% PAA-Gel of the transcription samples containing from left to right: low range ssRNA-marker, transcription with unlabeled dNTPs, 15N-UTP, 15N-GTP, 2F-ATP, 15N-UTP-GTP + 2F-ATP. Figure 1C: CD-melting curve of the Gswapt in the ligand bound state. The black curve represents the unlabeled RNA and the green curve represents the 19F-labeled RNA. The melting curves were recorded in the absence (left) and presence (right) of the ligand. Figure 1D: 1H-15N-HSQC of the Gswapt. The spectrum of the unlabeled RNA is shown on the top and the spectrum of the 19F-labeled RNA is shown at the bottom.

-20°C

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