DNase I activity measured in the presence of EDTA.
DNase I activity measured in the presence of EDTA.
2-Step DNase I Assay Kit
82213
Product group Assays
Overview
- SupplierBPS Bioscience
- Product Name2-Step DNase I Assay Kit
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe 2-Step DNase I Assay Kit is a homogeneous fluorescent based assay kit designed to measure DNase I activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant DNase I, DNA substrate, GroovyGreen™ dye, assay buffer and co-factors for 100 enzyme reactions. The kit also contains EDTA as internal control. Figure 1: 2-Step DNase I Assay Kit mechanism. DNase I cleaves DNA molecules into nucleotides. The amount of nucleotide formed correlates directly with DNase I activity and can be quantified by the addition of a fluorescent dsDNA-binding dye which distinguishes dsDNA from free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the signal is inversely proportional to DNase I activity
- UNSPSC41116133
