20-Hydroxyecdysone ELISA kit

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS|Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991|Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)|Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34|PRODUCT EXPLANATION: 20E KIT DEVELOPMENT AND SAMPLE PREPARATION|Porcheron P., Morinière M., Grassi J. and P. Pradelles. Development of an enzyme immunoassay for ecdysteroids using acetylcholinesterase as label. Insect Biochemistry, vol.19, No.2, p.117-122, 1989|Pascual N., Bellés X. et al. Quantification of ecdysteroids by immunoassay : comparison of enzyme immunoassay and radioimmunoassay. Zeitschrift für Naturforschung, 50c, 862-867, 1995|Claret J., Porcheron P. and Dray F. Critical period and ecdydone titers in the pupae of Pieris brassicae. Separatum Experientia 33, 1389, 1977|Porcheron P., Foucrier J., Gros Cl., Pradelles P.,et al. Radioimmunoassay of arthropod moulting hormone: β−ecdysone antibodies production and 125I-Iodinated tracer preparation. FEBS Letters, vol. 61, No.2, p.159, 1976|Porcheron P., Cassier P. and Dray F. Applications de techniques immunochimiques à l’étude de l’ecdysone. Bull. Soc. Zool. France, Suppl.1, p71-75, 1977|Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877|European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011|PRODUCT CITATIONS|Planelló R, Herrero Ó, Gómez-Sande P et al. Ecdysone-Related Biomarkers of Toxicity in the Model Organism Chironomus riparius: Stage and Sex-Dependent Variations in Gene Expression Profiles. PLoS One. 2015 Oct 8;10(10):e0140239|Christesen D, Yang YT,Somers J et al. Transcriptome Analysis of Drosophila melanogaster Third Instar Larval Ring Glands Points to Novel Functions and Uncovers a Cytochrome p450 Required for Development. G3: Genes, Genomes, Genetics (Bethesda), Vol. 7, No. 2, 2017, p. 467-479|Lafontaine A, Hanikenne M, Boulangé-Lecomte C et al. Vitellogenin and vitellogenin receptor gene expression and 20-hydroxyecdysone concentration in Macrobrachium rosenbergii exposed to chlordecone. Environ Sci Pollut Res Int. 2016 Oct;23(20):20661-20671|Li K, Tian L, Guo Z et al. 20-Hydroxyecdysone (20E) Primary Response Gene E75 Isoforms Mediate Steroidogenesis Autoregulation and Regulate Developmental Timing in Bombyx. J Biol Chem. 2016 Aug 26;291(35):18163-75|Gabrieli P, Kakani EG, Mitchell SN et al. Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae. Proc Natl Acad Sci U S A. 2014 Nov 18;111(46):16353-8|Koyama T, Rodrigues MA, Athanasiadis A et al. Nutritional control of body size through FoxO-Ultraspiracle mediated ecdysone biosynthesis. Elife. 2014 Nov 25;3