2,7-Difluorofluorescein diacetate

Chemodex

2,7-Difluorofluorescein diacetate [1027558-93-5]

10

1027558-93-5

Research Use Only

>97%

C24H14F2O7

452.4

2,7-Difluorofluorescein, also called Oregon Green 488 (OG488) dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line and useful for fluorescent labeling. It has a high extinction coefficient and fluorescence quantum yield and while it is pH insensitive in the physiological range, with a pKa of 4.6 it can be used to as a fluorescent indicator for intracellular pH and monitor pH changes in acidic organelles or pathways. OG488 is a fluorinated analog of fluoresceins. The absorption and emission spectra of OG488 perfectly match those of fluorescein. Because of the near match of the absorption maxima on proteins (~498 nm and ~512 nm) to the strong 488 nm and 514 nm spectral lines of the argon-ion laser, the Oregon Green 488 fluorphore is an important dye for both confocal laser-scanning microscopy and flow cytometry applications. Conjugates of OG488 are more photostable than those of fluorescein, allowing increased acquisition of photons before photodestruction of the dye and making it particularly a useful substitute for fluoresceins for fluorescence imaging applications. OG488 has a lower pKa (pKa = 4.7 versus 6.4 for fluorescein), making the fluorescence essentially pH insensitive in the physiological pH range. However, the pH sensitivity in the weakly acidic range (pH 4 to 6) also makes the dye useful as pH indicators for acidic organelles of live cells. This cell-permeable non-fluorescent 2,7-Difluorofluorescein diacetate is cleaved by cellular esterases and oxidation, and converted to the highly fluorescent 2,7-Difluorofluorescein. - Chemical. CAS: 1027558-93-5. Formula: C24H14F2O7. MW: 452.4. 2,7-Difluorofluorescein, also called Oregon Green 488 (OG488) dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line and useful for fluorescent labeling. It has a high extinction coefficient and fluorescence quantum yield and while it is pH insensitive in the physiological range, with a pKa of 4.6 it can be used to as a fluorescent indicator for intracellular pH and monitor pH changes in acidic organelles or pathways. OG488 is a fluorinated analog of fluoresceins. The absorption and emission spectra of OG488 perfectly match those of fluorescein. Because of the near match of the absorption maxima on proteins (~498 nm and ~512 nm) to the strong 488 nm and 514 nm spectral lines of the argon-ion laser, the Oregon Green 488 fluorphore is an important dye for both confocal laser-scanning microscopy and flow cytometry applications. Conjugates of OG488 are more photostable than those of fluorescein, allowing increased acquisition of photons before photodestruction of the dye and making it particularly a useful substitute for fluoresceins for fluorescence imaging applications. OG488 has a lower pKa (pKa = 4.7 versus 6.4 for fluorescein), making the fluorescence essentially pH insensitive in the physiological pH range. However, the pH sensitivity in the weakly acidic range (pH 4 to 6) also makes the dye useful as pH indicators for acidic organelles of live cells. This cell-permeable non-fluorescent 2,7-Difluorofluorescein diacetate is cleaved by cellular esterases and oxidation, and converted to the highly fluorescent 2,7-Difluorofluorescein.

O=C(O1)C2=C(C=CC=C2)C31C4=C(C=C(OC(C)=O)C(F)=C4)OC5=CC(OC(C)=O)=C(F)C=C53

-20°C,2°C to 8°C

41116134