4-Azido-L-homoalanine HCl (L-AHA)

Biorbyt

4-Azido-L-homoalanine HCl (L-AHA) [942518-29-8]

16

Proteins synthesis monitoring.

942518-29-8

Research Use Only

Theoretical MW: 144.13 g/mol; Detected MW: 144.06 g/mol

4-Azido-L-homoalanine (L-AHA) provides a non-radioactive alter-native to analyze the global protein synthesis in cell culture. It is cell-permeable and randomly incorporated instead of methionine during translation. The resulting azide-labeled full-length pro-teins can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging.

2°C to 8°C

12352200