ICC/IF analysis of HEK293 cells treated(B,D,F,H) or untreated(A,C,E,G) with 50 microM H?O? using GTX175714 Hydroxynonenal antibody [12F7]. Green : Primary antibody Red : F-Actin Blue : DAPI Dilution : 1:50 for 30-60 min at RT Fixation : 5% Formaldehyde for 5 min
![WB analysis of 4-hydroxy-nonenal-BSA using GTX175714 Hydroxynonenal antibody [12F7]. Lane 1 : Molecular Weight Ladder (MW) Lane 2 : BSA (0.5 microg) Lane 3 : 4-hydroxyl nonenal-BSA (0.5 microg) Lane 4 : 4-hydroxy nonenal-BSA (2.0 microg) Lane 5 : 4-hydroxy-2-hexenal (0.5 microg) Lane 6 : 4-hydroxy-2-hexenal (2.0 microg) Dilution : 1:1000 for 2 hours at RT](https://www.genetex.com/upload/website/prouct_img/normal/GTX17571/GTX17571_20180516_WB_w_23060620_255.webp "WB analysis of 4-hydroxy-nonenal-BSA using GTX175714 Hydroxynonenal antibody [12F7]. Lane 1 : Molecular Weight Ladder (MW) Lane 2 : BSA (0.5 microg) Lane 3 : 4-hydroxyl nonenal-BSA (0.5 microg) Lane 4 : 4-hydroxy nonenal-BSA (2.0 microg) Lane 5 : 4-hydroxy-2-hexenal (0.5 microg) Lane 6 : 4-hydroxy-2-hexenal (2.0 microg) Dilution : 1:1000 for 2 hours at RT")
ICC/IF analysis of HEK293 cells treated(B,D,F,H) or untreated(A,C,E,G) with 50 microM H?O? using GTX175714 Hydroxynonenal antibody [12F7]. Green : Primary antibody Red : F-Actin Blue : DAPI Dilution : 1:50 for 30-60 min at RT Fixation : 5% Formaldehyde for 5 min
4 Hydroxynonenal antibody [12F7]
GTX17571
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product Name4 Hydroxynonenal antibody [12F7]
- Delivery Days Customer9
- Antibody SpecificityDoes not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehy
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:50. ELISA: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID12F7
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- HostMouse
- IsotypeIgG1
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis. Chen JW et al., 2022, Front Cell NeurosciRead more