ChIP analysis of 2 microg of J1 ES genomic DNA spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications using GTX60801 5-Carboxylcytosine / 5-caC antibody. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IPd DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control.
ChIP analysis of 2 microg of J1 ES genomic DNA spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications using GTX60801 5-Carboxylcytosine / 5-caC antibody. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IPd DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control.
5-Carboxylcytosine / 5-caC antibody
GTX60801
ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product Name5-Carboxylcytosine / 5-caC antibody
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:500. IP: 1-5 microg. Dot: 1:500 - 1:1,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, ImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Insulin and Metformin Control Cell Proliferation by Regulating TDG-Mediated DNA Demethylation in Liver and Breast Cancer Cells. Yan JB et al., 2020 Sep 25, Mol Ther OncolyticsRead more