5-Ethynyl-dUTP (5-EdUTP)

Biorbyt

5-Ethynyl-dUTP (5-EdUTP)

16

Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Ventexo-or KOD XL). Incorporation into DNA by Primer Extension with family A polymerase Taqand family B polymerases (Pwo, Deep Vent exo-or KOD XL). The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: lambdamax 288 nm, epsilon 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Research Use Only

100 mM-110 mM

≥95%

Theoretical MW: 492.16 g/mol (free acid); Detected MW: 491.97 g/mol (free acid)

5-Ethynyl-dUTP (5-EdUTP)

-20°C

12352200