5-Ethynyl-UTP (5-EUTP)

Biorbyt

5-Ethynyl-UTP (5-EUTP)

16

Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription. The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin); to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes); to crosslink the RNA to azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: lambdamax 288 nm, epsilon 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Research Use Only

100 mM-110 mM

≥95%

Theoretical MW: 508.16 g/mol (free acid); Detected MW: 507.97 g/mol (free acid)

5-Ethynyl-UTP (5-EUTP)

12352200