Dot blot analysis of 4 - 100 ng (equivalent of 5 to 0.2 pmol of C-bases) hmC, mC and C controls using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Dilution : 1:600
![FISH (Fluorescence in situ hybridization) analysis of metaphase chromosomes from HeLa cells (treated with 0.1 microg/ml colcemid for 1 - 2 hours) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Red : Primary antibody Blue : DAPI Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60803/GTX60803_20201117_FISH_174_w_23061123_910.webp "FISH (Fluorescence in situ hybridization) analysis of metaphase chromosomes from HeLa cells (treated with 0.1 microg/ml colcemid for 1 - 2 hours) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Red : Primary antibody Blue : DAPI Dilution : 1:1000")
![ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Red : Primary antibody Blue : DAPI Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60803/GTX60803_20201117_ICCIF_173_w_23061123_119.webp "ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Red : Primary antibody Blue : DAPI Dilution : 1:1000")
![ICC/IF analysis of 4% PFA-fixed mouse embryos cells fixed in the one-cellular stage (after blocking with PBS containing 2% BSA, the cells were treated with 2N HCl) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Both the maternal (M) and paternal (P) pronuclei are stained. Left : Primary antibody Right : Anti- single stranded DNA antibody Permeabilization : 0.5% Triton X-100 for 30 mins Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX60803/GTX60803_20201117_ICCIF_175_w_23061123_794.webp "ICC/IF analysis of 4% PFA-fixed mouse embryos cells fixed in the one-cellular stage (after blocking with PBS containing 2% BSA, the cells were treated with 2N HCl) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Both the maternal (M) and paternal (P) pronuclei are stained. Left : Primary antibody Right : Anti- single stranded DNA antibody Permeabilization : 0.5% Triton X-100 for 30 mins Dilution : 1:200")
![ICC/IF analysis of 2.5% PFA fixed U2OS cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Figure A : cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 microM mCTP (left) or with DAPI (right). Figure B : staining of the cells with the 5-mC antibody after incubation of the antibody with 50 microM hmCTP and with DAPI. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX60803/GTX60803_20201117_ICCIF_176_w_23061123_845.webp "ICC/IF analysis of 2.5% PFA fixed U2OS cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Figure A : cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 microM mCTP (left) or with DAPI (right). Figure B : staining of the cells with the 5-mC antibody after incubation of the antibody with 50 microM hmCTP and with DAPI. Dilution : 1:500")
Dot blot analysis of 4 - 100 ng (equivalent of 5 to 0.2 pmol of C-bases) hmC, mC and C controls using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Dilution : 1:600
5-Methylcytosine / 5-mC antibody [GT9076]
GTX60803
ApplicationsDot Blot, ImmunoFluorescence, ImmunoCytoChemistry, Other Application
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product Name5-Methylcytosine / 5-mC antibody [GT9076]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:1,000. Dot: 1:600. FISH: 1:200 - 1:1,000. MeDIP: 1-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, ImmunoCytoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT9076
- Concentration1.3 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203