5-Propargylamino-dCTP-Texas Red

Jena Bioscience

5-Propargylamino-dCTP-Texas Red

5

Research Use Only

5-Propargylamino-dCTP-Texas Red is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-TexasRed/dCTP ratio for PCR and Nick Translation: 20-30% Propargylamino-dCTP-TexasRed7/80-70% dCTP (PCR), 30-50% Propargylamino-dCTP-TexasRed/70-50% dCTP (Nick Translation) Please note: Protect the Dye-labeled dCTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dCTP/dCTP ratio is recommended.

-20°C

41116133