TOP: untransfected control; BOTTOM: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
TOP: untransfected control; BOTTOM: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
6X His tag antibody [HIS.H8]
GTX18184
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product Name6X His tag antibody [HIS.H8]
- Delivery Days Customer9
- Antibody SpecificityRecognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tags location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
- Application Supplier NoteRecommended Starting Dilutions: For WB: Use at a dilution of 1:1000-1:3000. For ICC/IF: Use at a dilution of 1:500-1:2000. For IP: Use at a dilution of 1:50-1:200. Optimal dilutions should be determined experimentally by the end user.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHIS.H8
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG2b
- Scientific DescriptionThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- A dual-targeting PDGFRbeta/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo. Mabry R et al., 2010 Jan-Feb, MAbsRead more