ICC/IF analysis of 293T cells expressing His-Tag nuclear protein X using GTX33607 6X His tag antibody [RM146].
![IP analysis of 293T cells expressing a His-Tag fusion protein using GTX33607 6X His tag antibody [RM146]. Lane 1 : Whole lysate control Lane 2 : IP by rabbit IgG control Lane 3 : IP by Primary antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX33607/GTX33607_20200909_IP_237_w_23060800_618.webp "IP analysis of 293T cells expressing a His-Tag fusion protein using GTX33607 6X His tag antibody [RM146]. Lane 1 : Whole lysate control Lane 2 : IP by rabbit IgG control Lane 3 : IP by Primary antibody")
![WB analysis of various sample lysayes using GTX33607 6X His tag antibody [RM146]. Lane 1 : 293T cells Lane 2 : 293T cells transfected with His-Tag fusion protein X Lane 3 : E. coli lysate without His-Tag Protein Y Lane 4 : E. coli lysate with His-Tag Protein Y Dilution : 0.2microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX33607/GTX33607_20200909_WB_251_w_23060800_950.webp "WB analysis of various sample lysayes using GTX33607 6X His tag antibody [RM146]. Lane 1 : 293T cells Lane 2 : 293T cells transfected with His-Tag fusion protein X Lane 3 : E. coli lysate without His-Tag Protein Y Lane 4 : E. coli lysate with His-Tag Protein Y Dilution : 0.2microg/ml")
ICC/IF analysis of 293T cells expressing His-Tag nuclear protein X using GTX33607 6X His tag antibody [RM146].
6X His tag antibody [RM146]
GTX33607
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product Name6X His tag antibody [RM146]
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
- Application Supplier NoteWB: 0.1 microg/mL - 1 microg/mL. ICC/IF: 0.5 microg/mL - 2 microg/mL. FACS: 0.5 microg/mL - 2 microg/mL. IP: 0.5 microg/mL - 2 microg/mL. IHC: 0.1 microg/mL - 1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM146
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203