Tissue/cell: rat brain tissue(left panel was injury,Right panel was normal); 4% Paraformaldehyde-fixed and paraffin-embedded; _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; _x000D_ Incubation: Anti-8-OHdG Polyclonal Antibody, Unconjugated (bs-1278R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue(left panel was injury,Right panel was normal); 4% Paraformaldehyde-fixed and paraffin-embedded; _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; _x000D_ Incubation: Anti-8-OHdG Polyclonal Antibody, Unconjugated (bs-1278R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
8-OHdG Polyclonal Antibody
BS-1278R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Other Species
Overview
- SupplierBioss Antibodies
- Product Name8-OHdG Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityHuman, Mouse, Rat, Other Species
- Storage Instruction-20°C
- UNSPSC12352203
References
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