AAV/DJ-CAMKIIA(0.4)-SACAS9

Vector Biolabs

AAV/DJ-CAMKIIA(0.4)-SACAS9

5

Other Application

Research Use Only

1E13 GC/ml

Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. The main problem with Cas9 from Streptococcus pyogenes SF370 (SpCas9) is that this gene is too big to be packaged into AAV under most promoters, and theres no extra room for packaging a sgRNA along with the spCas9 in AAV. A small Cas9 from Staphylococcus aureus subsp. aureus (SaCas9) with high efficiency for cleaving mammalian endogenous DNA becomes the ideal solution to package into AAV for effective gene modification in vivo. This is an AAV-DJ (a synthetic serotype made from 8 wild type AAV including AAV2, 4, 5, 8, 9) expressing spCas9 nuclease under a neuronal specific CamKIIa promoter. The short CamKIIa promoter was derived from murine a-Calcium/calmodulin-dependent kinase II (CaMKII), a gene with expression restricted to excitatory neurons in the neocortex and hippocampus. This AAV-CamKIIa-saCas9 can be package into other AAV serotypes upon request.

-80°C

41106621