Western Blot of Goat anti-Uricase Peroxidase Conjugated antibody. Lane 1: purified Uricase under reducing conditions. Lane 2: purified Uricase under reducing conditions. Lane 3: purified Uricase under non-reducing conditions. Lane 4: purified Uricase under non-reducing conditions Load: ~1.0 microg (Lane 1 and 3) and 0.25 microg (Lane 2 and 4). Primary antibody: Uricase Peroxidase Conjugated Antibody at 1:5,000 overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 58 kDa, 58 kDa for Uricase. Other band(s): Uricase splice variants and isoforms.