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ABCB1 / MDR1 / P Glycoprotein Antibody (clone PG-13, Sepharose)

LS-C171189
LifeSpan BioSciences
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetABCB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ABCB1 / MDR1 / P Glycoprotein Antibody (clone PG-13, Sepharose)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation
  • Applications Supplier
    IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PG-13
  • Conjugate
    Sepharose
  • Estimated Purity
    ...
  • Gene ID5243
  • Target name
    ABCB1
  • Target description
    ATP binding cassette subfamily B member 1
  • Target synonyms
    ABC20; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-dependent translocase ABCB1; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; GP170; MDR1; multidrug resistance protein 1; p-170; P-glycoprotein 1; P-GP; PGY1; phospholipid transporter ABCB1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P Glycoprotein using anti-P Glycoprotein antibody (A00049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein antigen affinity purified polyclonal antibody (Catalog # A00049-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P Glycoprotein at approximately 160KD. The expected band size for P Glycoprotein is at 141KD.
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