ABCC2 / MRP2 Antibody (clone M2III-5, Concentrated)

LifeSpan BioSciences

ABCC2 / MRP2 Antibody (clone M2III-5, Concentrated)

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M2III-5 reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder character

M2III-5 has potential value for detection of MRP2-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-50 dilution. on acetone fixed cytospin preparations. For immunohistochemistry: M2III-5 (use 1:20) on acetone fixed frozen sections can be followed by incubation with rabbit anti-mouse IgG (1:25, Dako) and a monoclonal mouse APAAP complex (1:50, Dako). M2III-5 can also be used on formaldehyde-fixed paraffin-embedded human tissues and tumors, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 5 min 3 times. After incubation of M2III-5 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution, and anti-mouse-HRP.. Flo, ICC, IHC, IHC-Fr, IHC-P, WB M2III-5 has potential value for detection of MRP2-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-50 dilution. on acetone fixed cytospin preparations. For immunohistochemistry: M2III-5 (use 1:20) on acetone fixed frozen sections can be followed by incubation with rabbit anti-mouse IgG (1:25, Dako) and a monoclonal mouse APAAP complex (1:50, Dako). M2III-5 can also be used on formaldehyde-fixed paraffin-embedded human tissues and tumors, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 5 min 3 times. After incubation of M2III-5 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution, and anti-mouse-HRP.

Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

M2III-5

Unconjugated

...

ABCC2

ATP binding cassette subfamily C member 2

ABC30; ATP-binding cassette sub-family C member 2; ATP-binding cassette, sub-family C (CFTR/MRP), member 2; canalicular multidrug resistance protein; canalicular multispecific organic anion transporter 1; CMOAT; cMRP; DJS; MRP2; multidrug resistance-associated protein 2

Mouse

IgG2b

Human

2°C to 8°C

12352203