ABCC6 / MRP6 Antibody (clone M6II-31, Concentrated)

LifeSpan BioSciences

ABCC6 / MRP6 Antibody (clone M6II-31, Concentrated)

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M6II-31 reacts with MRP6, a 190-200 kD transmembrane protein that is related to the multidrug resistance related protein MRP. Mutations in the MRP6 gene are responsible for the connective tissue disorder Pseudoxanthoma elasticum (PXE). M6II-31 was raised

M6II-31 can be used to detect human MRP6 in cells and tissues. Immunocytochemistry: use 1:20-50 dilution on acetone fixed cytospin preparations. For immunohistochemistry: M6II-31 (use 1:20-50) on acetone fixed frozen sections can be followed by incubation with rabbit anti-rat IgG (1:25, Dako) and an APAAP complex (1:50, Dako). M6II-31 can be used on formaldehyde-fixed paraffin-embedded human tissues, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 10 minutes. After incubation of M6II-31 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-rat IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution and anti-rat-HRP.. Flo, IHC, IHC-Fr, IHC-P, WB M6II-31 can be used to detect human MRP6 in cells and tissues. Immunocytochemistry: use 1:20-50 dilution on acetone fixed cytospin preparations. For immunohistochemistry: M6II-31 (use 1:20-50) on acetone fixed frozen sections can be followed by incubation with rabbit anti-rat IgG (1:25, Dako) and an APAAP complex (1:50, Dako). M6II-31 can be used on formaldehyde-fixed paraffin-embedded human tissues, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 10 minutes. After incubation of M6II-31 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-rat IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution and anti-rat-HRP.

Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

M6II-31

Unconjugated

...

ABCC6

ATP binding cassette subfamily C member 6

ABC34; anthracycline resistance-associated protein; ARA; ATP-binding cassette sub-family C member 6; ATP-binding cassette, sub-family C (CFTR/MRP), member 6; EST349056; GACI2; MLP1; MOATE; MOAT-E; MRP6; multidrug resistance-associated protein 6; multi-specific organic anion transporter E; PXE; PXE1; URG7; URG7 protein

Rat

IgG2a

Human

2°C to 8°C

12352203