ChIP analysis of 4x10? LNCaP cells using GTX80693 Acetyl Lysine antibody [1C6]. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.
![WB analysis of untreated (DMSO only) or cells treated with 0.3uM or 3uM of Trichostatin A (TSA) for 16 hours using GTX80693 Acetyl Lysine antibody [1C6]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX80693/GTX80693_2133_WB_w_23061322_995.webp "WB analysis of untreated (DMSO only) or cells treated with 0.3uM or 3uM of Trichostatin A (TSA) for 16 hours using GTX80693 Acetyl Lysine antibody [1C6]. Dilution : 1:1000")
![ICC/IF analysis of HeLa cells untreated (left panels) or treated with 0.3uM Trichostatin A for 16 hours (right panels) using GTX80693 Acetyl Lysine antibody [1C6]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes](https://www.genetex.com/upload/website/prouct_img/normal/GTX80693/GTX80693_897_ICC-IF_w_23061322_630.webp "ICC/IF analysis of HeLa cells untreated (left panels) or treated with 0.3uM Trichostatin A for 16 hours (right panels) using GTX80693 Acetyl Lysine antibody [1C6]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes")
ChIP analysis of 4x10? LNCaP cells using GTX80693 Acetyl Lysine antibody [1C6]. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.
Acetyl Lysine antibody [1C6]
GTX80693
Product group Antibodies
Overview
- SupplierGeneTex
- Product NameAcetyl Lysine antibody [1C6] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:100 - 1:500. IP: 3 microg. ChIP assay: 10 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- Clone ID1C6
- Concentration1 mg/ml
- FormulationLiquid
- Scientific DescriptionIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1. Hung YH et al., 2022, Am J Cancer ResRead more
- HDAC6 involves in regulating the lncRNA-microRNA-mRNA network to promote the proliferation of glioblastoma cells. Wu AC et al., 2022 Feb 2, J Exp Clin Cancer ResRead more
- Upregulation of Znf179 acetylation by SAHA protects cells against oxidative stress. Wu CC et al., 2018 Oct, Redox BiolRead more