AChE antibody [HL1102] detects AChE protein at dendrite by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636298/GTX636298_44410_20210820_WB_M_tissue_w_23061202_168.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636298/GTX636298_44410_20220429_WB_brain_w_23061202_685.webp "Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636298/GTX636298_44410_20210903_WB_M_R_competitor_watermark_w_23061202_919.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![AChE antibody [HL1102] detects AChE protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636298/GTX636298_44410_20220321_ICC_IF_w_23061202_825.webp "AChE antibody [HL1102] detects AChE protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636298/GTX636298_45131_20230811_WB_M_R_25012200_637.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![AChE antibody [HL1102] detects AChE protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse nucleus accumbens. AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636298/GTX636298_45278_20251114_IHC-P_M_3_25112100_318.webp "AChE antibody [HL1102] detects AChE protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse nucleus accumbens. AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![AChE antibody [HL1102] detects AChE protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse basal forebrain. AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636298/GTX636298_45278_20251114_IHC-P_M_2_25112100_304.webp "AChE antibody [HL1102] detects AChE protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse basal forebrain. AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![AChE antibody [HL1102] detects AChE protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636298/GTX636298_45278_20251114_IHC-P_M_1_25112100_786.webp "AChE antibody [HL1102] detects AChE protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
AChE antibody [HL1102] detects AChE protein at dendrite by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
AChE antibody [HL1102]
GTX636298
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFeline, Human, Mouse, Rat
TargetACHE
Overview
- SupplierGeneTex
- Product NameAChE antibody [HL1102]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1102
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID43
- Target nameACHE
- Target descriptionacetylcholinesterase (Yt blood group)
- Target synonymsACEE, ARACHE, N-ACHE, YT, acetylcholinesterase, Yt blood group, acetylcholinesterase (Cartwright blood group), apoptosis-related acetylcholinesterase
- HostRabbit
- IsotypeIgG
- Protein IDP22303
- Protein NameAcetylcholinesterase
- Scientific DescriptionAcetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
- ReactivityFeline, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![ICC/IF analysis of Neuro-2a Cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22803/GTX22803_476_ICC-IF_w_23060620_619.webp)
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