IHC-P analysis of human Brain tissue using GTX22803 AChE antibody [HR2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human cerebellum tissue using GTX22803 AChE antibody [HR2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX22803/GTX22803_1131_IHC-P_w_23060620_833.webp "IHC-P analysis of human cerebellum tissue using GTX22803 AChE antibody [HR2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50")
![IHC-P analysis of human rectum tissue using GTX22803 AChE antibody [HR2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX22803/GTX22803_1132_IHC-P_w_23060620_807.webp "IHC-P analysis of human rectum tissue using GTX22803 AChE antibody [HR2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20")
![ICC/IF analysis of HeLa cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22803/GTX22803_475_ICC-IF_w_23060620_926.webp "ICC/IF analysis of HeLa cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of Neuro-2a Cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22803/GTX22803_476_ICC-IF_w_23060620_619.webp "ICC/IF analysis of Neuro-2a Cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of U251 cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22803/GTX22803_477_ICC-IF_w_23060620_199.webp "ICC/IF analysis of U251 cells using GTX22803 AChE antibody [HR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human Brain tissue using GTX22803 AChE antibody [HR2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
AChE antibody [HR2]
GTX22803
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Feline, Guinea Pig, Human, Mouse, Rabbit
TargetACHE
Overview
- SupplierGeneTex
- Product NameAChE antibody [HR2]
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not cross react with rat or frog AChE, and does not detect butyrylcholinesterase (BChE). Immunohistochemical staining of AChE in human brain samples with GTX22803 results in staining of nerve fibers and terminals.
- Application Supplier NoteICC/IF: 1:100-1:1000. IHC-Fr: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHR2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID43
- Target nameACHE
- Target descriptionacetylcholinesterase (Cartwright blood group)
- Target synonymsACEE; acetylcholinesterase; acetylcholinesterase (Yt blood group); apoptosis-related acetylcholinesterase; ARACHE; N-ACHE; YT; Yt blood group
- HostMouse
- IsotypeIgG2b
- Protein IDP22303
- Protein NameAcetylcholinesterase
- Scientific DescriptionAcetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]
- ReactivityBovine, Feline, Guinea Pig, Human, Mouse, Rabbit
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- In vivo localization of human acetylcholinesterase-derived species in a beta-sheet conformation at the core of senile plaques in Alzheimers disease. Jean L et al., 2019 Apr 19, J Biol ChemRead more
- Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure. Dafferner AJ et al., 2017 Oct 16, Chem Res ToxicolRead more