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Cross-reactivity assessment of ACP5 Mouse Recombinant Antibody AE00100 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of ACP5 Mouse Recombinant Antibody AE00100 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of ACP5 Mouse Recombinant Antibody AE00100 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.

ACP5 Recombinant Antibody AE00100

AE00100
Aeonian Biotech
TargetACP5
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Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    ACP5 Recombinant Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    IHC, PA
  • Category Supplier
    Antibody
  • Clone ID
    rACP5/1070
  • Gene ID54
  • Target name
    ACP5
  • Target description
    acid phosphatase 5, tartrate resistant
  • Target synonyms
    HPAP; human purple acid phosphatase; tartrate-resistant acid ATPase; tartrate-resistant acid phosphatase 5a; tartrate-resistant acid phosphatase 5b; tartrate-resistant acid phosphatase type 5; TRACP5a; TRACP5b; TRAP; TrATPase
  • Protein IDP13686
  • Protein Name
    Tartrate-resistant acid phosphatase type 5
  • Scientific Description
    ACP5 Recombinant Antibody AE00100
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SHKBP1 using anti-SHKBP1 antibody (A14104-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHKBP1 antigen affinity purified polyclonal antibody (Catalog # A14104-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHKBP1 at approximately 76 kDa. The expected band size for SHKBP1 is at 76 kDa.
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Figure 1. Western blot analysis of NLRP9 using anti-NLRP9 antibody (A14119-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP9 antigen affinity purified polyclonal antibody (Catalog # A14119-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP9 at approximately 113 kDa. The expected band size for NLRP9 is at 113 kDa.
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