
HUVEC cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Acrosin Polyclonal Antibody(bs-5151R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Acrosin Polyclonal Antibody
BS-5151R

ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Goat, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetACR
Overview
- SupplierBioss Antibodies
- Product NameAcrosin Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID49
- Target nameACR
- Target descriptionacrosin
- Target synonymsacrosin; acrosin light and heavy chain prepropeptide; preproacrosin; proacrosin
- HostRabbit
- IsotypeIgG
- Protein IDP10323
- Protein NameAcrosin
- ReactivityBovine, Canine, Equine, Goat, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
References
- In-vitro differentiation of early pig spermatogenic cells to haploid germ cells. Yu K et al., 2019 Sep 1, Mol Hum ReprodRead more
- Fluoride Interferes with the Sperm Fertilizing Ability via Downregulated SPAM1, ACR, and PRSS21 Expression in Rat Epididymis. Liu Y et al., 2019 May 8, J Agric Food ChemRead more
- Melatonin up-regulates the expression of the GATA-4 transcription factor and increases testosterone secretion from Leydig cells through RORalpha signaling in an in vitro goat spermatogonial stem cell differentiation culture system. Deng SL et al., 2017 Dec 15, OncotargetRead more