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ACRP / SPAG7 Antibody (N-Terminus, HRP)

LS-C474345
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSPAG7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACRP / SPAG7 Antibody (N-Terminus, HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.65 mg/ml
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID9552
  • Target name
    SPAG7
  • Target description
    sperm associated antigen 7
  • Target synonyms
    ACRP; FSA-1; sperm-associated antigen 7
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPAG7 using anti-SPAG7 antibody (A14828-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPAG7 antigen affinity purified polyclonal antibody (Catalog # A14828-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPAG7 at approximately 35 kDa. The expected band size for SPAG7 is at 26 kDa.
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