ICC/IF analysis of fresh mouse sperms using GTX00690 ADAM1B antibody [#158]. Dilution : 1:300 Fixation : 4% PFA
![WB analysis of mouse testis lysates collected from different ages using GTX00690 ADAM1B antibody [#158]. ADAM1B appeared as a faint signal at 3 weeks of age.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00690/GTX00690_20191104_WB_1_w_23053121_979.webp "WB analysis of mouse testis lysates collected from different ages using GTX00690 ADAM1B antibody [#158]. ADAM1B appeared as a faint signal at 3 weeks of age.")
![WB analysis of various mouse tissue samples using GTX00690 ADAM1B antibody [#158]. The Adam1B synthesized in testis (120 kDa) is proteolytically processed into smaller form (60 kDa) in sperm during the epididymal maturation of the spermatozoa. By western blotting, ADAM1B is detected in testis extract at 120 kDa position, which is due to glycosylation and larger than predicted size of 89 kDa from the sequence. This protien is testis-specific. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00690/GTX00690_20191104_WB_w_23053121_793.webp "WB analysis of various mouse tissue samples using GTX00690 ADAM1B antibody [#158]. The Adam1B synthesized in testis (120 kDa) is proteolytically processed into smaller form (60 kDa) in sperm during the epididymal maturation of the spermatozoa. By western blotting, ADAM1B is detected in testis extract at 120 kDa position, which is due to glycosylation and larger than predicted size of 89 kDa from the sequence. This protien is testis-specific. Dilution : 1:100")
ICC/IF analysis of fresh mouse sperms using GTX00690 ADAM1B antibody [#158]. Dilution : 1:300 Fixation : 4% PFA
ADAM1B antibody [#158] - Orthogonal Validated
GTX00690
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityMouse
TargetAdam1b
Overview
- SupplierGeneTex
- Product NameADAM1B antibody [#158] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody showed no reactivity with ADAM1A (confirmed by WB using recombinant ADAM1A and ADAM1B proteins).
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID#158
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID280667
- Target nameAdam1b
- Target descriptiona disintegrin and metallopeptidase domain 1b
- Target synonymsa disintegrin and metalloproteinase domain 1b; ADAM 1b; Adam1; disintegrin and metalloproteinase domain-containing protein 1b; fertilin alpha; fertilin subunit alpha-b; Ft; Ftna; PH-30 alpha
- HostRat
- IsotypeIgG2a
- ReactivityMouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Mice expressing aberrant sperm-specific protein PMIS2 produce normal-looking but fertilization-incompetent spermatozoa. Yamaguchi R et al., 2012 Jul, Mol Biol CellRead more
- Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected]. Tokuhiro K et al., 2012 Mar 6, Proc Natl Acad Sci U S ARead more
- Calsperin is a testis-specific chaperone required for sperm fertility. Ikawa M et al., 2011 Feb 18, J Biol ChemRead more