ICC/IF analysis of a representative time-lapse view of sperm-egg fusion using GTX00691 ADAM1B antibody [#57]. The initiation of fusion (time 0) was detected by transfer of Hoechst 33342 dye to sperm in the same area (indicated by arrowheads). The diffusion of membrane in the postacrosomal area identified by ADAM1B (green) started about 60 s after the initiation of sperm-egg fusion and was accompanied by expansion of the Hoechst 33342 staining area toward the posterior head.
ADAM1B antibody [#57] - Orthogonal Validated
GTX00691
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse
TargetAdam1b
Overview
- SupplierGeneTex
- Product NameADAM1B antibody [#57] - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID#57
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID280667
- Target nameAdam1b
- Target descriptiona disintegrin and metallopeptidase domain 1b
- Target synonymsa disintegrin and metalloproteinase domain 1b; ADAM 1b; Adam1; disintegrin and metalloproteinase domain-containing protein 1b; fertilin alpha; fertilin subunit alpha-b; Ft; Ftna; PH-30 alpha
- HostRat
- IsotypeIgG2a
- ReactivityMouse
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1. Satouh Y et al., 2012 Nov 1, J Cell SciRead more