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ADAMTS13 Antibody (YA2002)

HY-P82257
MedChem Express
TargetADAMTS13
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ADAMTS13 Antibody (YA2002)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID11093
  • Target name
    ADAMTS13
  • Target description
    ADAM metallopeptidase with thrombospondin type 1 motif 13
  • Target synonyms
    A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; ADAM-TS13; ADAMTS-13; C9orf8; von Willebrand factor-cleaving protease; vWF-cleaving protease; VWFCP; vWF-CP
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ADAMTS13 Antibody (YA2002) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2002), targeting ADAMTS13, with a predicted molecular weight of 154 kDa (observed band size: 154 kDa). ADAMTS13 Antibody (YA2002) can be used for WB, FC experiment in human background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody (A00586-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS13 antigen affinity purified polyclonal antibody (Catalog # A00586-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS13 at approximately 154 kDa. The expected band size for ADAMTS13 is at 154 kDa.
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