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ADAR2 antibody

GTX114237
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetADARB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ADAR2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID104
  • Target name
    ADARB1
  • Target description
    adenosine deaminase RNA specific B1
  • Target synonyms
    ADAR2; adenosine deaminase, RNA-specific, B1 (homolog of rat RED1); double-stranded RNA-specific editase 1; DRABA2; DRADA2; dsRNA adenosine deaminase DRADA2; NEDHYMS; RED1; RNA editing deaminase 1; RNA-editing enzyme 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78563
  • Protein Name
    Double-stranded RNA-specific editase 1
  • Scientific Description
    This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing. Freund EC et al., 2020 May 19, Cell Rep
    Read more
  • Androgen receptor-regulated circFNTA activates KRAS signaling to promote bladder cancer invasion. Chen J et al., 2020 Apr 3, EMBO Rep
    Read more
  • Systematic characterization of A-to-I RNA editing hotspots in microRNAs across human cancers. Wang Y et al., 2017 Jul, Genome Res
    Read more
  • Reduced adenosine-to-inosine miR-455-5p editing promotes melanoma growth and metastasis. Shoshan E et al., 2015 Mar, Nat Cell Biol
    Read more

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Figure 1. Western blot analysis of RED1/ADARB1 using anti-RED1/ADARB1 antibody (A01810-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RED1/ADARB1 antigen affinity purified polyclonal antibody (Catalog # A01810-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RED1/ADARB1 at approximately 90 kDa. The expected band size for RED1/ADARB1 is at 81 kDa.
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