Adenovirus Fiber trimer antibody [2A6]

GeneTex

Adenovirus Fiber trimer antibody [2A6]

9

Recognizes only trimers (high molecular weight bands 180 - 200kDa) on Western blots of unboiled Ad2 and Ad5 fiber proteins and shows immunofluorescence with Ad5 fiber mutant H5ts142 infected cells at 32ºC but not at 39.5ºC.

For IF: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry

Research Use Only

Monoclonal

2A6

0.2 mg/ml

Unconjugated

Mouse

IgG2a

Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms beta sheet and beta bends, and a knob at the C terminus that contains the type specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the virion.

Virus

2°C to 8°C

12352203