Bio-Connect

ADENOVIRUS-GFP-FLPO

1776
Vector Biolabs
ApplicationsOther Application
Product group Expression
200 ul
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Overview

  • Supplier
    Vector Biolabs
  • Product Name
    ADENOVIRUS-GFP-FLPO
  • Delivery Days Customer
    5
  • Applications
    Other Application
  • Certification
    Research Use Only
  • Concentration
    1E10 PFU/ml
  • Scientific Description
    FLP from Saccharomyces cerevisiae, a site-specific recombinases (SSR) from the lambda integrase family, recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. This mouse codon optimized FLPe greatly increase the recombination efficiency in mouse cells. This adenovirus express a mouse codon-optimized FLP recombinase (FLPo), along with an eGFP marker. FLPo and eGFP are driven by the same CMV promoter and separated by a 2A peptide.
  • Storage Instruction
    -80°C
  • UNSPSC
    41106621