ADENOVIRUS-MCHERRY-FLAG-HCAS9

Vector Biolabs

ADENOVIRUS-MCHERRY-FLAG-HCAS9

5

Other Application

Research Use Only

1E10 PFU/ml

Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This adenovirus expresses a codon-optimized Cas9 nuclease, along with a GFP reporter. It can be used along with guided RNA for genome-editing. This adenovirus expresses a FLAG tagged Cas9 with a mCherry reporter.

-80°C

41106621