ADENOVIRUS-RFP-FLPO

Vector Biolabs

ADENOVIRUS-RFP-FLPO

5

Other Application

Research Use Only

1E10 PFU/ml

FLP is a site-specific recombinases (SSR) from the lambda integrase family, which recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency in mouse cells. This adenovirus express FLPo, along with a RFP marker. FLPo and RFP are driven by the same CMV promoter and separated by a 2A peptide.

-80°C

41106621