FACS analysis of HeLa cells using GTX22769 AHR antibody Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature[RPT9] compared to an isotype control (blue).
![FACS analysis of NIH/3T3 cells using GTX22769 AHR antibody [RPT9] compared to an isotype control (blue). Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22769/GTX22769_136_FACS_w_23060620_499.webp "FACS analysis of NIH/3T3 cells using GTX22769 AHR antibody [RPT9] compared to an isotype control (blue). Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![FACS analysis of PC-3 cells using GTX22769 AHR antibody [RPT9] compared to an isotype control (blue). Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22769/GTX22769_137_FACS_w_23060620_870.webp "FACS analysis of PC-3 cells using GTX22769 AHR antibody [RPT9] compared to an isotype control (blue). Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![ICC/IF analysis of HeLa Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22769/GTX22769_20190918_ICC-IF_1_w_23060620_322.webp "ICC/IF analysis of HeLa Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of U251 Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22769/GTX22769_20190918_ICC-IF_2_w_23060620_488.webp "ICC/IF analysis of U251 Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![WB analysis of 20 microg of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (Lane B) or empty vector control (Lane A) using GTX22769 AHR antibody [RPT9]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22769/GTX22769_2072_WB_w_23060620_295.webp "WB analysis of 20 microg of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (Lane B) or empty vector control (Lane A) using GTX22769 AHR antibody [RPT9]. Dilution : 1:1000")
![ICC/IF analysis of A2058 Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22769/GTX22769_413_ICC-IF_w_23060620_836.webp "ICC/IF analysis of A2058 Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
FACS analysis of HeLa cells using GTX22769 AHR antibody Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature[RPT9] compared to an isotype control (blue).
AHR antibody [RPT9]
GTX22769
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAhr
Overview
- SupplierGeneTex
- Product NameAHR antibody [RPT9]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:10-1:100. FACS: 1:50. ChIP assay: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRPT9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID25690
- Target nameAhr
- Target descriptionaryl hydrocarbon receptor
- Target synonymsah receptor; aryl hydrocarbon receptor
- HostMouse
- IsotypeIgG3
- Scientific Descriptionbinds 3-methylcholanthrene and other aromatic hydrocarbons; may play a role in bone formation [RGD, Feb 2006]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203