Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![AKT antibody [N3C2], Internal detects AKT protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX121937/GTX121937_44741_20220916_ICC_IF_22110201_201.webp "AKT antibody [N3C2], Internal detects AKT protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![AKT antibody [N3C2], Internal detects AKT protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX121937/GTX121937_44776_20221028_IHC-P_22122722_902.webp "AKT antibody [N3C2], Internal detects AKT protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. AKT stained by AKT antibody [N3C2], Internal (GTX121937) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX121937/GTX121937_43264_20180706_WB_M_w_23060519_816.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 μg of Akt1/2/3 antibody [N3C2], Internal (GTX121937). Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GTX121937). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX121937/GTX121937_40450_20150327_IP_w_23060519_222.webp "Immunoprecipitation of Akt1/2/3 protein from 293T whole cell extracts using 5 μg of Akt1/2/3 antibody [N3C2], Internal (GTX121937). Western blot analysis was performed using Akt1/2/3 antibody [N3C2], Internal (GTX121937). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX121937/GTX121937_43264_20180824_WB_B_w_23060519_793.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT antibody [N3C2], Internal (GTX121937) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
AKT antibody [N3C2], Internal
GTX121937
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFish, Human, Mouse, Porcine, Rat
TargetAKT1
Overview
- SupplierGeneTex
- Product NameAKT antibody [N3C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.67 mg/ml
- ConjugateUnconjugated
- Gene ID207
- Target nameAKT1
- Target descriptionAKT serine/threonine kinase 1
- Target synonymsAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1
- HostRabbit
- IsotypeIgG
- Scientific DescriptionThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
- ReactivityFish, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Binh DHN, Cheng TC, Tu SH, et al. StAR-related lipid transfer domain protein 3 (STARD3) regulates HER2 and promotes HER2-positive breast cancer progression through interaction with HSP90 and SRC signaling. Am J Cancer Res. 2023,13(11):5151-5173.Read this paper
- Sulistyowati E, Huang SE, Cheng TL, et al. Vasculoprotective Potential of Baicalein in Angiotensin II-Infused Abdominal Aortic Aneurysms through Inhibiting Inflammation and Oxidative Stress. Int J Mol Sci. 2023,24(21). doi: 10.3390/ijms242116004Read this paper
- Wang P, Gao Y, Yang G, et al. Enhancing the inhibition of cell proliferation and induction of apoptosis in H22 hepatoma cells through biotransformation of notoginsenoside R1 by Lactiplantibacillus plantarum S165 into 20(S/R)-notoginsenoside R2. RSC Adv. 2023,13(42):29773-29783. doi: 10.1039/d3ra06029bRead this paper
- Du HX, Wang H, Ma XP, et al. Eukaryotic translation initiation factor 2α kinase 2 in pancreatic cancer: An approach towards managing clinical prognosis and molecular immunological characterization. Oncol Lett. 2023,26(5):478. doi: 10.3892/ol.2023.14066Read this paper
- Liang W, Gao Y, Zhao Y, et al. Lactiplantibacillus plantarum ELF051 Alleviates Antibiotic-Associated Diarrhea by Regulating Intestinal Inflammation and Gut Microbiota. Probiotics Antimicrob Proteins. 2024,16(6):1996-2006. doi: 10.1007/s12602-023-10150-xRead this paper
- Pérez-Mora S, Ocampo-López J, Gómez-García MDC, et al. BFNB Enhances Hair Growth in C57BL/6 Mice through the Induction of EGF and FGF7 Factors and the PI3K-AKT-β-Catenin Pathway. Int J Mol Sci. 2023,24(15). doi: 10.3390/ijms241512110Read this paper
- Huang C, Lin ZJ, Chen JC, et al. α-Viniferin-Induced Apoptosis through Downregulation of SIRT1 in Non-Small Cell Lung Cancer Cells. Pharmaceuticals (Basel). 2023,16(5). doi: 10.3390/ph16050727Read this paper
- Russo M, Moccia S, Luongo D, et al. Senolytic Flavonoids Enhance Type-I and Type-II Cell Death in Human Radioresistant Colon Cancer Cells through AMPK/MAPK Pathway. Cancers (Basel). 2023,15(9). doi: 10.3390/cancers15092660Read this paper
- Yang NC, Chin CY, Zheng YX, et al. The Attenuation of Insulin/IGF-1 Signaling Pathway Plays a Crucial Role in the Myo-Inositol-Alleviated Aging in Caenorhabditis elegans. Int J Mol Sci. 2023,24(7). doi: 10.3390/ijms24076194Read this paper
- Li Y, Nie J, Deng C, et al. P-15 promotes chondrocyte proliferation in osteoarthritis by regulating SFPQ to target the Akt-RUNX2 axis. J Orthop Surg Res. 2023,18(1):199. doi: 10.1186/s13018-023-03658-zRead this paper
Datasheet
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