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ProductsBack/AKT1/2/3 (Thr305/308/309) Antibody
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates probed with phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. The upper band could be the fully glycosylated form of the target protein.
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates probed with phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. The upper band could be the fully glycosylated form of the target protein.
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates probed with phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Polyclonal Antibody, Unconjugated (bs-5182R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. The upper band could be the fully glycosylated form of the target protein.

AKT1/2/3 (Thr305/308/309) Antibody

BS-5182R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetAKT1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    AKT1/2/3 (Thr305/308/309) Antibody
  • Delivery Days Customer
    16
  • Antibody Specificity
    The antibody recognizes phosphorylation at Thr308 of AKT1 and because of sequence similarity, it will also detect phosphorylation of Thr309 of AKT2 and phosphorylation at Thr305 of AKT3.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID207
  • Target name
    AKT1
  • Target description
    AKT serine/threonine kinase 1
  • Target synonyms
    AKT; AKT1m; PKB; PKB alpha; PKB-ALPHA; PRKBA; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y243
  • Protein Name
    RAC-gamma serine/threonine-protein kinase
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Aspirin inhibits adipogenesis of tendon stem cells and lipids accumulation in rat injury tendon through regulating PTEN/PI3K/AKT signalling. Wang Y et al., 2019 Nov, J Cell Mol Med
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