Alkaline Phosphatase Staining Kit

Cosmo Bio USA

Alkaline Phosphatase Staining Kit

16

Research Use Only

Alkaline Phosphatase Staining Kit is designed for the rapid and sensitive detection of osteoblasts differentiated in culture from bone marrow cells. Bone forming osteoblasts and bone-degrading osteoclasts can be distinguished by the expression, respectively, of alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP). CosmoBios TRAP staining kit (PMC-AK04F-COS) is ideal for rapid and convenient detection of the latter. Simple staining protocol <ol> Remove culture medium. Wash each well 3 times with 1mL PBS. Add 500microL of 10% formalin neutral buffer solution to each well and fix for 20 minutes at room temperature. Remove 10% formalin neutral buffer solution and wash each well 3 times with 2mL deionized water. Dissolve 1 vial of Chromogenic Substrate in 5mL Substrate-containing Buffer. Add 400microL Chromogenic Substrate to each well. Incubate at 37°C for 5-20 minutes. Adjust incubation time until stained. Wash with deionized water to stop the reaction. </ol> References <ol> A. S. H. De Jong, M. Van Kessel-Van Vark and A. K. Raap. Sensitivity of various visualization methods for peroxidase and alkaline phosphatase activity in immunoenzyme histochemistry. The Histochemical Journal Volume 17, 1119-1130 (1985) </ol>

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