
WB analysis of various sample lysates using GTX03390 Alkaline Phosphatase (Tissue Non-Specific) antibody. Lane 1 : HeLa whole cell lysates Lane 2 : HEK293 whole cell lysates Lane 3 : rat kidney tissue lysates Lane 4 : RH-35 whole cell lysates Lane 5 : mouse kidney tissue lysates Lane 6 : HEPA1-6 whole cell lysates Loading : 50μg Dilution : 0.5 μg/mL
Alkaline Phosphatase (Tissue Non-Specific) antibody
GTX03390
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALPL
Overview
- SupplierGeneTex
- Product NameAlkaline Phosphatase (Tissue Non-Specific) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 0.25-0.5microg/ml. ICC/IF: 5microg/ml. ELISA: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Gene ID249
- Target nameALPL
- Target descriptionalkaline phosphatase, biomineralization associated
- Target synonymsAP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, liver/bone/kidney-type alkaline phosphatase, phosphoamidase, phosphocreatine phosphatase, tissue non-specific alkaline phosphatase, tissue-nonspecific ALP
- HostRabbit
- IsotypeIgG
- Protein IDP05186
- Protein NameAlkaline phosphatase, tissue-nonspecific isozyme
- Scientific DescriptionThis gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203


![IHC-P analysis of ovarian cancer tissue using GTX60568 Alkaline Phosphatase (Tissue Non-Specific) antibody [2F4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60568/GTX60568_20170912_IHC-P_w_23061123_541.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637904/GTX637904_T-44872_20230303_WB_multiple_B_23030717_526.webp)




