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ProductsBack/Alpha 1 Acid Glycoprotein (ORM1) Rabbit Polyclonal Antibody
Western blot of ORM1/Alpha-1-acid glycoprotein antibody. R1044P. Lane 1: Human AGP1. Load: 50 ng. Primary antibody: Anti human ORM1/AGP1 antibody at 1/1,000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted at 24 kDa / Observed at 40 kDa; protein migrates at 40 kDa.
Western blot of ORM1/Alpha-1-acid glycoprotein antibody. R1044P. Lane 1: Human AGP1. Load: 50 ng. Primary antibody: Anti human ORM1/AGP1 antibody at 1/1,000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted at 24 kDa / Observed at 40 kDa; protein migrates at 40 kDa.
Western blot of ORM1/Alpha-1-acid glycoprotein antibody. R1044P. Lane 1: Human AGP1. Load: 50 ng. Primary antibody: Anti human ORM1/AGP1 antibody at 1/1,000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted at 24 kDa / Observed at 40 kDa; protein migrates at 40 kDa.

Alpha 1 Acid Glycoprotein (ORM1) Rabbit Polyclonal Antibody

R1044PS
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetORM1
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Alpha 1 Acid Glycoprotein (ORM1) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5004
  • Target name
    ORM1
  • Target description
    orosomucoid 1
  • Target synonyms
    AGP 1; AGP1; AGP-A; alpha-1-acid glycoprotein 1; epididymis secretory sperm binding protein Li 153w; HEL-S-153w; OMD 1; ORM
  • Host
    Rabbit
  • Protein IDP02763
  • Protein Name
    Alpha-1-acid glycoprotein 1
  • Scientific Description
    Alpha 1 Acid Glycoprotein (ORM1) rabbit polyclonal antibody, Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.
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