IHC-P analysis of human heart tissue using GTX22807 alpha Adaptin antibody [AC1-M11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![WB analysis of tissue and membrane enriched extract (30 ug) of Rat Brain (Lane 1), SH-SY5Y (Lane 2), U-87 MG (Lane 3), COLO 205 (Lane 4), PC-3 (Lane 5), MCF7 (Lane 6), NIH/3T3 (Lane 7) and A-431 (Lane 8) using GTX22807 alpha Adaptin antibody [AC1-M11]. Dilution : 2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX22807/GTX22807_1602_WB_w_23060620_478.webp "WB analysis of tissue and membrane enriched extract (30 ug) of Rat Brain (Lane 1), SH-SY5Y (Lane 2), U-87 MG (Lane 3), COLO 205 (Lane 4), PC-3 (Lane 5), MCF7 (Lane 6), NIH/3T3 (Lane 7) and A-431 (Lane 8) using GTX22807 alpha Adaptin antibody [AC1-M11]. Dilution : 2 microg/ml")
![ICC/IF analysis of SH-SY5Y cells using GTX22807 alpha Adaptin antibody [AC1-M11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22807/GTX22807_483_ICC-IF_w_23060620_312.webp "ICC/IF analysis of SH-SY5Y cells using GTX22807 alpha Adaptin antibody [AC1-M11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of NRK cells using GTX22807 alpha Adaptin antibody [AC1-M11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22807/GTX22807_484_ICC-IF_w_23060620_811.webp "ICC/IF analysis of NRK cells using GTX22807 alpha Adaptin antibody [AC1-M11].")
IHC-P analysis of human heart tissue using GTX22807 alpha Adaptin antibody [AC1-M11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
alpha Adaptin antibody [AC1-M11]
GTX22807
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAmphibian, Bovine, Chicken, Human, Mouse, Porcine, Primate, Rat
TargetAP2A1
Overview
- SupplierGeneTex
- Product Namealpha Adaptin antibody [AC1-M11]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.
- Application Supplier NoteWB: 2 microg/mL. ICC/IF: 2 microg/mL. IHC-P: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAC1-M11
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID784636
- Target nameAP2A1
- Target descriptionadaptor related protein complex 2 subunit alpha 1
- Target synonymsadaptor related protein complex 2 alpha 1 subunit; AP-2 complex subunit alpha-1
- HostMouse
- IsotypeIgG2a
- ReactivityAmphibian, Bovine, Chicken, Human, Mouse, Porcine, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability. Ganapathy AS et al., 2022 Sep, AutophagyRead more
- Phosphorylation by Dyrk1A of clathrin coated vesicle-associated proteins: identification of the substrate proteins and the effects of phosphorylation. Murakami N et al., 2012, PLoS OneRead more