More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Alpha fetoprotein / AFP antibody [2A9]
ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].

Alpha fetoprotein / AFP antibody [2A9]

GTX84954
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse
TargetAFP
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Alpha fetoprotein / AFP antibody [2A9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. ICC/IF: 1:100. IHC-P: 1:150. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID174
  • Target name
    AFP
  • Target description
    alpha fetoprotein
  • Target synonyms
    AFPD, FETA, HPAFP, alpha-fetoprotein, alpha-1-fetoprotein, alpha-fetoglobulin
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP02771
  • Protein Name
    Alpha-fetoprotein
  • Scientific Description
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Reactivity
    Canine, Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chen YH, Su CC, Deng W, et al. Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells. Sci Rep. 2019,9(1):9919. doi: 10.1038/s41598-019-46359-6
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of alpha 1 Fetoprotein/AFP using anti-alpha 1 Fetoprotein/AFP antibody (A00522-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-alpha 1 Fetoprotein/AFP antigen affinity purified polyclonal antibody (Catalog # A00522-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for alpha 1 Fetoprotein/AFP at approximately 69 kDa. The expected band size for alpha 1 Fetoprotein/AFP is at 69 kDa.
Product group Antibodies
Boster Bio
Anti-alpha 1 Fetoprotein/AFP Antibody Picoband(r)A00522-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAFP
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Alpha-fetoprotein (AFP) [AF5]AB00213-1.1-BT
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
ReactivityHuman
TargetAFP
  • SizePrice
Product group Antibodies
Abbexa
Alpha-Fetoprotein (AFP) AntibodyABX000589
ApplicationsWestern Blot
  • SizePrice
Immunohistochemical staining of human liver shows moderate positivity in secretion.
Product group Antibodies
Atlas Antibodies
Anti-AFP AntibodyAMAB91610
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetAFP
  • SizePrice
Product group Antibodies
Biosynth
AFP antibody10-1003
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Human AFP Antibody130-10547
ApplicationsELISA
TargetAFP
  • SizePrice
Product group Antibodies
Biomatik
AFP Polyclonal AntibodyCAC14116
ApplicationsWestern Blot, ELISA
TargetAFP
  • SizePrice
Product group Antibodies
Assay Genie
Alpha-Fetoprotein (AFP) AntibodyCAB0200
ReactivityHuman
TargetAFP
  • SizePrice
Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
Product group Antibodies
References
Bioss
AFP (A2) Monoclonal AntibodyBSM-1621M
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Rat
TargetAFP
  • SizePrice
Western blot analysis of HepG2, diluted at 1:2,000.
Product group Antibodies
Cusabio
AFP Monoclonal AntibodyCSB-MA000233
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAFP
  • SizePrice