ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
![IHC-P analysis of human liver tissue using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84954/GTX84954_3277_IHC-P_w_23061420_659.webp "IHC-P analysis of human liver tissue using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![WB analysis of 3T3 cell lysate using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84954/GTX84954_3589_WB_v2_w_23061420_510.webp "WB analysis of 3T3 cell lysate using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with Alpha fetoprotein / AFP plasmid (Right) or empty vector (Left) for 48 hrs using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84954/GTX84954_4738_WB_w_23061420_312.webp "WB analysis of HEK293T cells transfected with Alpha fetoprotein / AFP plasmid (Right) or empty vector (Left) for 48 hrs using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84954/GTX84954_4739_WB_w_23061420_832.webp "WB analysis of various cell lines using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Loading : 35 ug per lane")
![FACS analysis of Jurkat cells using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84954/GTX84954_636_FACS_w_23061420_924.webp "FACS analysis of Jurkat cells using GTX84954 Alpha fetoprotein / AFP antibody [2A9]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of HEK293T cells transfected with either Alpha fetoprotein / AFP plasmid(Red) or empty vector control plasmid(Blue) using GTX84954 Alpha fetoprotein / AFP antibody [2A9].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84954/GTX84954_637_FACS_w_23061420_517.webp "FACS analysis of HEK293T cells transfected with either Alpha fetoprotein / AFP plasmid(Red) or empty vector control plasmid(Blue) using GTX84954 Alpha fetoprotein / AFP antibody [2A9].")
ICC/IF analysis of COS7 cells transiently transfected with Alpha fetoprotein / AFP plasmid using GTX84954 Alpha fetoprotein / AFP antibody [2A9].
Alpha fetoprotein / AFP antibody [2A9]
GTX84954
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse
TargetAFP
Overview
- SupplierGeneTex
- Product NameAlpha fetoprotein / AFP antibody [2A9] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ICC/IF: 1:100. IHC-P: 1:150. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID174
- Target nameAFP
- Target descriptionalpha fetoprotein
- Target synonymsAFPD; alpha-1-fetoprotein; alpha-fetoglobulin; alpha-fetoprotein; FETA; HPAFP
- HostMouse
- IsotypeIgG2b
- Protein IDP02771
- Protein NameAlpha-fetoprotein
- Scientific DescriptionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
- ReactivityCanine, Human, Monkey, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Mitochondrial Akt Signaling Modulated Reprogramming of Somatic Cells. Chen YH et al., 2019 Jul 9, Sci RepRead more