WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at 1 microg/ml.
alpha Tubulin antibody [DM1A]
GTX27291
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Chicken, Fungus, Human, Monkey, Mouse, Rat, Yeast
Overview
- SupplierGeneTex
- Product Namealpha Tubulin antibody [DM1A]
- Delivery Days Customer9
- Antibody SpecificityThe antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.
- Application Supplier NoteWB: 0.5-1 microg/ml. ICC/IF: 0.5-1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDDM1A
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- HostMouse
- IsotypeIgG1
- Scientific DescriptionTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.
- ReactivityBovine, Canine, Chicken, Fungus, Human, Monkey, Mouse, Rat, Yeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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