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WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at 1 microg/ml.
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at 1 microg/ml.
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at 1 microg/ml.

alpha Tubulin antibody [DM1A]

Research Use Only
GTX27291
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Chicken, Fungus, Human, Monkey, Mouse, Rat, Yeast
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Overview

  • Supplier
    GeneTex
  • Product Name
    alpha Tubulin antibody [DM1A]
  • Delivery Days Customer
    9
  • Antibody Specificity
    The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.
  • Application Supplier Note
    WB: 0.5-1 microg/ml. ICC/IF: 0.5-1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DM1A
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Formulation
    Liquid
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.
  • Reactivity
    Bovine, Canine, Chicken, Fungus, Human, Monkey, Mouse, Rat, Yeast
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

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  • BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins. Young AM et al., 2020 Jul 15, Mol Biol Cell
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  • PGRMC2 is an intracellular haem chaperone critical for adipocyte function. Galmozzi A et al., 2019 Dec, Nature
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  • NPGPx-Mediated Adaptation to Oxidative Stress Protects Motor Neurons from Degeneration in Aging by Directly Modulating O-GlcNAcase. Hsieh YL et al., 2019 Nov 19, Cell Rep
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  • Dengue virus-elicited tryptase induces endothelial permeability and shock. Rathore AP et al., 2019 Jul 2, J Clin Invest
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  • Targeting PKCdelta as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer. Lee PC et al., 2018 Dec 10, Cancer Cell
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  • HSPB7 is indispensable for heart development by modulating actin filament assembly. Wu T et al., 2017 Nov 7, Proc Natl Acad Sci U S A
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  • Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via beta-hydroxybutyrate. Huang CK et al., 2017 Mar 10, Nat Commun
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  • The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion. Chou SF et al., 2015 Oct, PLoS Pathog
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  • Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling. Lee PT et al., 2014 Dec 1, Nucleic Acids Res
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