Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C).Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alpha-Tubulin, mAb (rec.) (F2C) (1microg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
![alpha Tubulin antibody [F2C]](https://www.genetex.com/upload/website/prouct_img/normal/GTX33912/GTX33912_20160831_Structure_w_23060800_118.webp "alpha Tubulin antibody [F2C]")
Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C).Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alpha-Tubulin, mAb (rec.) (F2C) (1microg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
alpha Tubulin antibody [F2C]
GTX33912
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse
Overview
- SupplierGeneTex
- Product Namealpha Tubulin antibody [F2C]
- Delivery Days Customer7
- Antibody SpecificityRecognizes mouse, bovine and human α-tubulin.
- Application Supplier NoteImmunocytochemistry: 1:1000, Western Blot: 1:1000
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDF2C
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostHuman
- IsotypeIgG2
- ReactivityBovine, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203