Aminoallyl-dUTP-Cy3

Biorbyt

Aminoallyl-dUTP-Cy3

16

Incorporation into DNA/cDNA by: Primer Extension with Klenow fragment; PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: lambdaexc 550 nm, lambdaem 570 nm, epsilon 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), CF 2600.08.

Research Use Only

1.0-1.1 mM

Liquid

Aminoallyl-dUTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-Cy3/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-Cy3/50% dTTP.

-20°C

12352200