Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Antibody (bs-4002R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Antibody (bs-4002R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
AMPK alpha-1/2 (Thr183/Thr172) Antibody
BS-4002R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat, Sheep
Overview
- SupplierBioss Antibodies
- Product NameAMPK alpha-1/2 (Thr183/Thr172) Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
References
- MSTN Attenuates Cardiac Hypertrophy through Inhibition of Excessive Cardiac Autophagy by Blocking AMPK /mTOR and miR-128/PPARgamma/NF-kappaB. Qi H et al., 2020 Mar 6, Mol Ther Nucleic AcidsRead more
- Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Zhang W et al., 2018 Mar, ChemosphereRead more
- PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome. Saito Y et al., 2017 Nov 23, Sci RepRead more
- Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways. Zhao Y et al., 2016 Nov 24, Sci RepRead more
- Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis. Wang Y et al., 2016 Dec 15, Toxicol Appl PharmacolRead more