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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Antibody (bs-4002R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Antibody (bs-4002R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with AMPK alpha-1/2 (Thr183/Thr172) Antibody (bs-4002R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

AMPK alpha-1/2 (Thr183/Thr172) Antibody

BS-4002R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetPRKAA1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    AMPK alpha-1/2 (Thr183/Thr172) Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5562
  • Target name
    PRKAA1
  • Target description
    protein kinase AMP-activated catalytic subunit alpha 1
  • Target synonyms
    5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK alpha 1; AMPK subunit alpha-1; AMPK, alpha, 1; AMPKa1; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; tau-protein kinase PRKAA1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • MSTN Attenuates Cardiac Hypertrophy through Inhibition of Excessive Cardiac Autophagy by Blocking AMPK /mTOR and miR-128/PPARgamma/NF-kappaB. Qi H et al., 2020 Mar 6, Mol Ther Nucleic Acids
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  • Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Zhang W et al., 2018 Mar, Chemosphere
    Read more
  • PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome. Saito Y et al., 2017 Nov 23, Sci Rep
    Read more
  • Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways. Zhao Y et al., 2016 Nov 24, Sci Rep
    Read more
  • Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis. Wang Y et al., 2016 Dec 15, Toxicol Appl Pharmacol
    Read more