ProductsBack/AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody

Untreated (-) and treated (+) MCF-7 whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody

GTX52341

ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Product group Antibodies

ReactivityHuman, Mouse, Rat

TargetPRKAA1

Overview

Supplier
GeneTex
Product Name
AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody
Delivery Days Customer
9
Application Supplier Note
WB 1:500-1:2000, IHC 1:50-1:200
Applications
ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Polyclonal
Concentration
1 mg/ml
Conjugate
Unconjugated
Gene ID5562
Target name
PRKAA1
Target description
protein kinase AMP-activated catalytic subunit alpha 1
Target synonyms
AMPK, AMPK alpha 1, AMPKa1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase, catalytic alpha-1 chain, ACACA kinase, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK subunit alpha-1, AMPK, alpha, 1, HMGCR kinase, acetyl-CoA carboxylase kinase, hydroxymethylglutaryl-CoA reductase kinase, protein kinase, AMP-activated, alpha 1 catalytic subunit, tau-protein kinase PRKAA1
Host
Rabbit
Isotype
IgG
Protein IDQ13131
Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
Scientific Description
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Reactivity
Human, Mouse, Rat
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

References

Vadhan A, Yang YF, Wang YM, et al. Fumarate hydratase inhibits non-small cell lung cancer metastasis via inactivation of AMPK and upregulation of DAB2. Oncol Lett. 2023,25(1):42. doi: 10.3892/ol.2022.13627
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Tseng HI, Zeng YS, Lin YJ, et al. A novel AMPK activator shows therapeutic potential in hepatocellular carcinoma by suppressing HIF1α-mediated aerobic glycolysis. Mol Oncol. 2022,16(11):2274-2294. doi: 10.1002/1878-0261.13211
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Lu KY, Primus Dass KT, Lin SZ, et al. N-butylidenephthalide ameliorates high-fat diet-induced obesity in mice and promotes browning through adrenergic response/AMPK activation in mouse beige adipocytes. Biochim Biophys Acta Mol Cell Biol Lipids. 2021,1866(12):159033. doi: 10.1016/j.bbalip.2021.159033
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Zuliani I, Lanzillotta C, Tramutola A, et al. High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimer's Disease Signatures. Int J Mol Sci. 2021,22(7). doi: 10.3390/ijms22073746
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Kang DH, Jeong DJ, Ahn TS, et al. Expression of AMP-activated protein kinase/ten-eleven translocation 2 and their clinical relevance in colorectal cancer. Oncol Lett. 2021,21(2):164. doi: 10.3892/ol.2021.12425
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Yang Q, Ma Q, Xu J, et al. Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders. Front Cell Dev Biol. 2020,8:611354. doi: 10.3389/fcell.2020.611354
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Zuliani I, Lanzillotta C, Tramutola A, et al. The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome. Neurotherapeutics. 2021,18(1):340-363. doi: 10.1007/s13311-020-00978-4
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Simanjuntak Y, Ko HY, Lee YL, et al. Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice. PLoS Pathog. 2020,16(5):e1008521. doi: 10.1371/journal.ppat.1008521
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Lu CC, Chiang JH, Tsai FJ, et al. Metformin triggers the intrinsic apoptotic response in human AGS gastric adenocarcinoma cells by activating AMPK and suppressing mTOR/AKT signaling. Int J Oncol. 2019,54(4):1271-1281. doi: 10.3892/ijo.2019.4704
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Yang Q, Xu J, Ma Q, et al. PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis. Nat Commun. 2018,9(1):4667. doi: 10.1038/s41467-018-07132-x
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