Untreated (-) and treated (+) MCF-7 whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (-) and treated (+) MCF-7 whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody
GTX52341
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKAA1
Overview
- SupplierGeneTex
- Product NameAMPK alpha (phospho Thr172) antibody - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThe sequence around AMPK alpha 1 (phospho Thr183) is conserved with the sequence around AMPK alpha 2 (phospho Thr172).AAPK1
- Application Supplier NoteWB 1:500-1:2000, IHC 1:50-1:200
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5562
- Target namePRKAA1
- Target descriptionprotein kinase AMP-activated catalytic subunit alpha 1
- Target synonyms5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK alpha 1; AMPK subunit alpha-1; AMPK, alpha, 1; AMPKa1; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 1 catalytic subunit; tau-protein kinase PRKAA1
- HostRabbit
- IsotypeIgG
- Protein IDQ13131
- Protein Name5'-AMP-activated protein kinase catalytic subunit alpha-1
- Scientific DescriptionThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Fumarate hydratase inhibits non-small cell lung cancer metastasis via inactivation of AMPK and upregulation of DAB2.Read more
- A novel AMPK activator shows therapeutic potential in hepatocellular carcinoma by suppressing HIF1alpha-mediated aerobic glycolysis. Tseng HI et al., 2022 Jun, Mol OncolRead more
- N-butylidenephthalide ameliorates high-fat diet-induced obesity in mice and promotes browning through adrenergic response/AMPK activation in mouse beige adipocytes. Lu KY et al., 2021 Dec, Biochim Biophys Acta Mol Cell Biol LipidsRead more
- High-Fat Diet Leads to Reduced Protein O-GlcNAcylation and Mitochondrial Defects Promoting the Development of Alzheimers Disease Signatures. Zuliani I et al., 2021 Apr 3, Int J Mol SciRead more
- Expression of AMP-activated protein kinase/ten-eleven translocation 2 and their clinical relevance in colorectal cancer. Kang DH et al., 2021 Feb, Oncol LettRead more
- Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders. Yang Q et al., 2020, Front Cell Dev BiolRead more
- The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome. Zuliani I et al., 2021 Jan, NeurotherapeuticsRead more
- Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice. Simanjuntak Y et al., 2020 May, PLoS PathogRead more
- Metformin triggers the intrinsic apoptotic response in human AGS gastric adenocarcinoma cells by activating AMPK and suppressing mTOR/AKT signaling. Lu CC et al., 2019 Apr, Int J OncolRead more
- PRKAA1/AMPKalpha1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis. Yang Q et al., 2018 Nov 7, Nat CommunRead more