ANO1 / DOG1 / TMEM16A Antibody (clone tDAN4)
LS-C391827
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetANO1
Overview
- SupplierLifeSpan BioSciences
- Product NameANO1 / DOG1 / TMEM16A Antibody (clone tDAN4)
- Delivery Days Customer23
- Antibody SpecificityHuman ANO1 / DOG1 / TMEM16A
- Application Supplier NoteExpression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG11 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%. Titering of the DOG1 antibody may be required for optimal performance. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.. IHC-P (0.5 - 1 µg/ml) Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG11 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%. Titering of the DOG1 antibody may be required for optimal performance. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
- ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDtDAN4
- Concentration0.2 mg/ml
- ConjugateUnconjugated
- Estimated Purity...
- Gene ID55107
- Target nameANO1
- Target descriptionanoctamin 1
- Target synonymsanoctamin 1, calcium activated chloride channel; anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; discovered on gastrointestinal stromal tumors protein 1; DOG1; oral cancer overexpressed 2; ORAOV2; TAOS2; TMEM16A; transmembrane protein 16A (eight membrane-spanning domains); tumor-amplified and overexpressed sequence 2
- HostMouse
- IsotypeIgG1
- ReactivityHuman
- Storage Instruction2°C to 8°C
- UNSPSC12352203