Bio-Connect

ANO1 / DOG1 / TMEM16A Antibody (clone tDAN4)

LS-C391827
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetANO1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ANO1 / DOG1 / TMEM16A Antibody (clone tDAN4)
  • Delivery Days Customer
    23
  • Antibody Specificity
    Human ANO1 / DOG1 / TMEM16A
  • Application Supplier Note
    Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG11 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%. Titering of the DOG1 antibody may be required for optimal performance. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.. IHC-P (0.5 - 1 µg/ml) Expression of TMEM16A (DOG1) protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG11 has been reported in 97.8 percent of scorable GISTs, including all c-kit negative GISTs. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha (PDGFR alpha) mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GISTs is nearly identical: 94.4% vs. 94.7%. Titering of the DOG1 antibody may be required for optimal performance. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    tDAN4
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55107
  • Target name
    ANO1
  • Target description
    anoctamin 1
  • Target synonyms
    anoctamin 1, calcium activated chloride channel; anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; discovered on gastrointestinal stromal tumors protein 1; DOG1; oral cancer overexpressed 2; ORAOV2; TAOS2; TMEM16A; transmembrane protein 16A (eight membrane-spanning domains); tumor-amplified and overexpressed sequence 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203