Anti-1-pHis [SC50-3]

Absolute Antibody

Anti-1-pHis [SC50-3]

7

This antibody is specific for 1-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers.

This antibody can be used for the identification of 1-pHis substrates and functional study of pHis. A dot blot was preformed on rabbit antisera, the 1-pTza, 3-pTza peptide libraries, His control library and a pTyr peptide with this antibody demonstrating its specificity to 1-pHis (Fuhs et al, 2015; pmid:26140597). This antibody has been also used for a western blot on His6-NDPK-A protein (Felix et al, 2020; pmid:32384736). Immunofluorescence was preformed on mouse tissues with this antibody (Yang et al, 2019; pmid:31721359). An ELISA was preformed on 3-day differentiated Sca1+-VPCs with this antibody (Yang et al, 2019; pmid:31721359). Ca1+-VPCs were lysed and immunoprecipitated with this clone (Yang et al, 2019; pmid:31721359). Finally, immunoaffinity purification (IAP) columns, containing protein A crosslinked with this antibody. The samples were SK-N-BE(2) xenograft tumors from mice (Adam et al, 2020; pmid:32392889).

Western Blot, ELISA, Purification/Extraction/Isolation

WB; ELISA; Immunoaffinity purification

Research Use Only

Monoclonal

SC50-3

Mouse

This chimeric Fab mouse fragment was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.

Other Species

Species independent

The original rabbit version of this antibody was raised by immunizing rabbits with a 1-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).

-20°C,2°C to 8°C

12352203