Figure 1. IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073). ALOX15 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073). ALOX15 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband(r)
PB9073-APC
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALOX15
Overview
- SupplierBoster Bio
- Product NameAnti-15 Lipoxygenase 1/ALOX15 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for ALOX15 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateAPC (Allophycocyanin)
- Gene ID246
- Target nameALOX15
- Target descriptionarachidonate 15-lipoxygenase
- Target synonyms12/15-lipoxygenase; 12-LOX; 15-lipooxygenase-1; 15-lipoxygenase type 1; 15-LOX; 15-LOX type 1; 15-LOX-1; arachidonate 12-lipoxygenase, leukocyte-type; arachidonate omega-6 lipoxygenase; hepoxilin A3 synthase Alox15; linoleate 13S-lipoxygenase; LOG15; polyunsaturated fatty acid lipoxygenase ALOX15
- HostRabbit
- IsotypeIgG
- Protein IDP16050
- Protein NameArachidonate 15-lipoxygenase
- Scientific DescriptionBoster Bio Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband® catalog # PB9073. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203